IgG ELISA for invasive amoebiasis in endemic areas
نویسندگان
چکیده
An IgG ELISA was evaluated as a serological test for the diagnosis of invasive amoebic disease in an endemic area. Sixteen patients with proven amoebic dysentery, 22 with suspected amoebic dysentery and 21 with amoebic liver abscesses were studied. Control subjects were 26 asymptomatic amoebic cyst passers, 13 patients with giardiasis, 26with enteric fever and 31 individuals with no evidence of parasitic infestation. The mean optical density (OD) value of the negative controls + 2 standard deviations (SD) was taken as the cut-off point to differentiate seropositive from seronegative individuals. It was found that all patients with amoebic liver abscesses had diagnostically raised IgG titres with specificity and sensitivity rates of 60.5% and 100% respectively; 25% of the patients with proven and 50% with suspected amoebiasis had positive antibody values with specificity and sensitivity rates of 50% and 25% respectively. Among the control subjects only 4 (15%) asymptomatic cyst passers, 4 (31%) patients with giardiasis, 3 (11%) of those with enteric fever and 5 (16%) of individuals without parasite infestation were seropositive. The results of this study indicate that the IgG ELISA often provides equivocal results in cases of amoebic dysentery in endemic areas. However, specific IgG titres are significantly raised and of value in the diagnosis of patients clinically suspected to have an amoebic liver abscess.
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